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Extracellular vesicles derived from Acholeplasma laidlawii PG8

Vladislav M Chernov1, Olga A Chernova, Alexey A Mouzykantov

  • 1Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russian Federation.

Thescientificworldjournal
|May 31, 2011
PubMed

Insights

Bacteria, including mycoplasmas like Acholeplasma laidlawii, produce extracellular vesicles. These vesicles carry genetic material and proteins, and can be mutagenic, with stress inducing their formation.

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Physiology

Background:

  • Extracellular vesicle (EV) production is common in bacteria.
  • Data on EV production in Mollicutes, such as mycoplasmas, is lacking.
  • Acholeplasma laidlawii is a ubiquitous mycoplasma and common cell culture contaminant.

Purpose of the Study:

  • To investigate extracellular vesicle production in Mollicutes.
  • To characterize the properties of vesicles produced by Acholeplasma laidlawii.
  • To determine if vesicle production is a common phenomenon in Mollicutes.

Main Methods:

  • Isolation and characterization of ultramicroforms (extracellular vesicles) from Acholeplasma laidlawii PG8.
  • Microscopy (size, morphology, ultrastructural organization).
  • Analysis of vesicle contents (genetic material, proteins) and biological activity (mutagenicity).

Main Results:

  • Acholeplasma laidlawii PG8 produces ultramicroforms similar to bacterial membrane vesicles.
  • These vesicles contain genetic material and proteins.
  • The vesicles demonstrated mutagenicity towards human peripheral blood lymphocytes.
  • Mycoplasma gallisepticum S6 also produces similar structures.
  • Stress conditions increase ultramicroform production in mycoplasmas.

Conclusions:

  • Extracellular vesicle production is likely a common phenomenon in Mollicutes.
  • Mycoplasma-derived vesicles possess genetic material, proteins, and biological activity.
  • Stress-induced vesicle formation suggests a role in mycoplasma adaptation or interaction.
  • Further research is needed to elucidate the precise function of these vesicles in mycoplasmas.

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