Related Experiment Video
Updated: Jun 1, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
Highlights of the optical highlighter fluorescent proteins
1Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, U.S.A.
None:
The development of super-resolution microscopy techniques using molecular localization, such as photoactivated localization microscopy, fluorescence photoactivated localization microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy with independent running acquisition and many others, has heightened interest in molecules that will be grouped here into a category referred to as 'optical highlighter' fluorescent proteins. This review will survey many of the advances in development of fluorescent proteins for optically highlighting sub-populations of fluorescently labelled molecules.
Related Concept Videos
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Super-resolution Fluorescence Microscopy
Fluorescence and Phosphorescence: Instrumentation
Confocal Fluorescence Microscopy
Immunofluorescence Microscopy
The...
Total Internal Reflection Fluorescence Microscopy
