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Peptide Identification Using Tandem Mass Spectrometry

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Absolute protein quantification by LC-ICP-MS using MeCAT peptide labeling.

Diego Esteban-Fernández¹, Christian Scheler, Michael W Linscheid

¹Department of Chemistry, Humboldt-Universitaet zu Berlin, Berlin, Germany. diesteban77@yahoo.es

Analytical and Bioanalytical Chemistry

|June 1, 2011

Summary

This study introduces a new method for absolute protein quantification using metal-coded affinity tag (MeCAT) labeling and inductively coupled plasma mass spectrometry (ICP-MS). This approach enhances sensitivity and accuracy in quantitative proteomics.

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Area of Science:

Proteomics
Analytical Chemistry
Mass Spectrometry

Background:

Current quantitative proteomics often relies on isotope-coded labeling and mass spectrometry.
Inductively coupled plasma mass spectrometry (ICP-MS) offers potential advantages like higher sensitivity and absolute quantification capabilities.
Lanthanide-based metal-coded affinity tag (MeCAT) reagents are emerging tools in elemental mass spectrometry for proteomics.

Purpose of the Study:

To develop and present one of the first methodologies for absolute quantification of peptides and proteins using MeCAT labeling.
To validate the use of MeCAT labeling coupled with ICP-MS for accurate protein quantification.

Main Methods:

Metal-coded affinity tag (MeCAT) labeling with lanthanides (specifically Europium-Eu).
Liquid chromatography (LC) separation interfaced with ICP-MS for quantification.
LC coupled with electrospray ionization mass spectrometry (ESI-MS) for identification.
Use of synthetic labeled peptides as external calibration standards.
Isotope dilution ICP-MS for precise quantification.

Main Results:

A robust methodology for absolute quantification of peptides and proteins using MeCAT-Eu labeling and ICP-MS was established.
External calibration curves were generated using multi-peptide mixes, accounting for chromatographic effects.
The quantification strategy was successfully applied to labeled peptides and standard proteins like digested lysozyme and bovine serum albumin.

Conclusions:

MeCAT labeling combined with ICP-MS provides a sensitive and accurate method for absolute quantification in proteomics.
This approach overcomes limitations of traditional methods, enabling more reliable protein analysis.
The developed methodology holds promise for advancing quantitative proteomics research.