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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Regulation of pre-miRNA processing.

Nicolas J Lehrbach1, Eric A Miska

  • 1Wellcome Trust Cancer Research UK Gurdon Institute and Department of Biochemistry, University of Cambridge, The Henry Wellcome Building of Cancer and Developmental Biology, Tennis Court Rd, Cambridge CB2 1QN, UK.

Advances in Experimental Medicine and Biology
|June 2, 2011
PubMed
Summary
This summary is machine-generated.

The RNA-binding protein Lin28/LIN-28 prevents processing of the let-7 microRNA precursor, controlling stem cell differentiation. This interaction regulates developmental pathways in C. elegans and mammals.

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Last Updated: Jun 1, 2026

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Published on: June 12, 2018

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • microRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
  • miRNA expression is critical for development and disease.
  • Post-transcriptional control mechanisms fine-tune miRNA levels.

Purpose of the Study:

  • To investigate the regulation of pre-miRNA processing by specific factors.
  • To focus on the role of RNA-binding protein Lin28/LIN-28 in controlling let-7 miRNA processing.
  • To analyze the function of the let-7/Lin28/LIN-28 axis in stem cell differentiation.

Main Methods:

  • Examining the interaction between Lin28/LIN-28 and pre-let-7 miRNA.
  • Investigating the Dicer-mediated processing of pre-let-7.
  • Analyzing the uridylation and degradation pathways of pre-let-7.
  • Studying the conserved function of let-7 and Lin28/LIN-28 in model organisms.

Main Results:

  • Lin28/LIN-28 sequesters pre-let-7 miRNA, inhibiting Dicer processing.
  • Interaction with Lin28/LIN-28 leads to pre-let-7 uridylation.
  • Uridylated pre-let-7 undergoes subsequent degradation.
  • The let-7/Lin28/LIN-28 pathway acts as a conserved developmental switch.

Conclusions:

  • Lin28/LIN-28 is a key regulator of let-7 miRNA biogenesis.
  • This regulatory mechanism controls stem cell differentiation.
  • The let-7/Lin28/LIN-28 axis plays a vital role in conserved developmental processes.