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Microbial Biosensors

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Updated: Jun 1, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor.

Oscar R Miranda¹, Xiaoning Li, Limary Garcia-Gonzalez

¹Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.

Journal of the American Chemical Society

|June 2, 2011

Summary

This summary is machine-generated.

A novel enzyme-nanoparticle system enables sensitive colorimetric detection of microbial contamination. This method quantifies bacteria in solution and on test strips, crucial for environmental and clinical pathogen detection.

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Colorimetric Detection of Bacteria Using Litmus Test

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Last Updated: Jun 1, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

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Published on: May 28, 2012

Colorimetric Detection of Bacteria Using Litmus Test

Colorimetric Detection of Bacteria Using Litmus Test

Published on: September 17, 2016

Area of Science:

Nanotechnology
Biochemistry
Microbiology

Background:

Rapid and sensitive pathogen detection is crucial for environmental and clinical applications.
Current methods may lack the sensitivity or speed required for certain settings.

Purpose of the Study:

To develop a colorimetric enzyme-nanoparticle conjugate system for microbial contamination detection.
To achieve sensitive and rapid quantification of bacteria using this novel approach.

Main Methods:

Utilized cationic gold nanoparticles (NPs) electrostatically bound to β-galactosidase (β-Gal) to inhibit enzyme activity.
Analyte bacteria binding to NPs released β-Gal, restoring enzyme activity for a colorimetric readout.
Developed a field-friendly test strip format for practical application.

Main Results:

Quantified bacteria at concentrations as low as 1 × 10(2) bacteria/mL in solution.
Achieved detection limits of 1 × 10(4) bacteria/mL using the test strip format.
Demonstrated an enzyme-amplified colorimetric signal proportional to bacterial presence.

Conclusions:

The enzyme-nanoparticle system offers a sensitive and rapid method for detecting microbial contamination.
This approach is adaptable for both solution-based assays and field-deployable test strips.
The technology holds promise for environmental monitoring and clinical diagnostics.