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Published on: August 24, 2011
Evaluation of a commercially available rapid urinary porphobilinogen test
Michael Vogeser1, Thomas Stauch
1Institute of Clinical Chemistry, University of Munich, Munich, Germany. michael.vogeser@med.uni-muenchen.de
Background:
Demonstration of substantially increased urinary excretion of porphobilinogen is the cornerstone of diagnosing acute porphyria crisis. Because porphobilinogen testing is not implemented on clinical chemistry analysers, respective analyses are available in rather few clinical laboratories. The aim of this study was to critically describe and to evaluate a semi-quantitative rapid test for urinary porphobilinogen determination which is commercially available and recommended by the American Porphyria Foundation.
Methods:
Urinary samples from patients with acute intermittent porphyria and control samples were analysed and the semi-quantitative results were compared with the results obtained by a manual quantitative spectrophotometric method.
Results:
In all 32 samples studied, acceptable agreement between the results of the rapid test and the quantitative test was observed. Handling of the test was found to be convenient.
Conclusions:
The assay was found to be reliable and has the potential to increase the availability of porphobilinogen testing in the field.
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