Cajanus indicus leaf protein: Beneficial role in experimental organ pathophysiology. A review

The herb, Cajanus indicus L, has been and is popular for its medicinal value in India and other countries for long. The herb is mainly cultivated for the seeds which are used as pulses and are rich in proteins. People of rural India and some neighboring countries use the aqueous extract of the leaves of the herb against poor liver function and recently it has been found that the extract is not only useful against liver damage but also beneficial for renal failure and a number of other pathophysiological conditions. Intraperitoneal administration of the aqueous protein fraction of the leaves has shown hepatoprotective activity in mice. The protein fraction revealed the presence of a 43kDa protein having antioxidant and other protective properties in organ pathophysiology. The purified protein, CI-protein, scavenges free radicals generated by different free radical inducers and helps providing cytoprotection. Amino acid sequence of CI-protein has some structural similarity with plastocyanin, an electron carrier protein in photosynthesis. The protein has also been found to be active against a number of organ dysfunction inducer chemicals and drugs, like carbon tetrachloride, thioacetamide and acetaminophen. Signal transduction studies suggest that CI-protein exerts its protective action by free radical scavenging and antioxidative properties; it activates NF-κB and Akt without any involvement of ERK1/ERK2 and STAT-3 in acetaminophen induced hepatic pathophysiology. Besides, it reduces both drug and toxin induced cytotoxicity by decreasing the formation and/or scavenging of free radicals involving cytochrome P450, taking part in detoxification of xenobiotics.