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Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

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Related Experiment Video

Updated: Jun 1, 2026

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
10:03

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Investigating human keratinocyte stem cell identity.

Loubna Chadli1, Michèle T Martin, Nicolas O Fortunel

  • 1Alternative Energies and Atomic Energy Commission, Institute of Cellular and Molecular Radiobiology, Laboratory of Genomics and Radiobiology of Keratinopoiesis, Evry Cedex, France. nicolas.fortunel@cea.fr

European Journal of Dermatology : EJD
|June 2, 2011
PubMed
Summary
This summary is machine-generated.

Human keratinocyte stem cells offer a unique model for studying skin regeneration and stemness. Developing methods for their prospective selection is crucial for advancing research in this accessible human tissue.

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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

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Related Experiment Videos

Last Updated: Jun 1, 2026

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
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Published on: January 30, 2021

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
08:36

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Published on: August 20, 2015

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
08:26

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Area of Science:

  • Dermatology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Adult stem cell research predominantly uses animal models.
  • The epidermis is an accessible human tissue, making keratinocyte stem cells a valuable research model.
  • The regenerative capacity of keratinocyte stem cells is clinically validated.

Purpose of the Study:

  • To dissect the genetic and epigenetic controls of stemness and self-renewal in human keratinocyte stem cells.
  • To identify tools for the prospective selection of homogeneous keratinocyte stem cells.
  • To advance the understanding of stem cell-associated functional characteristics.

Main Methods:

  • Leveraging studies from the broader stem cell field.
  • Employing targeted approaches for keratinocyte stem cell research.
  • Developing and utilizing functional assays for stem cell characterization.

Main Results:

  • Human keratinocyte stem cells are a unique system for studying stemness.
  • A significant challenge is obtaining homogeneous keratinocyte stem cell populations.
  • Advances require improved functional assays for stem cell assessment.

Conclusions:

  • Human keratinocyte stem cells are crucial for understanding epidermis regeneration.
  • Prospective selection tools are needed for homogeneous keratinocyte stem cell isolation.
  • Functional assays are key to advancing stem cell research in dermatology.