Related Experiment Video
Updated: Jun 1, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
Published on: June 19, 2015
Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive
Thu Betteridge1, Sally R Partridge, Jonathan R Iredell
1The iThree Institute, University of Technology, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia.
Abstract:
Most surveys for class 1 integrons are at least partly predicated on PCR screening that targets integron conserved regions. However, class 1 integrons are structurally diverse, so dependence on conserved regions may lead to missing clinically relevant examples of class 1 integrons. Here, we surveyed a commensal population of bacteria from patients in an intensive care unit to identify class 1 integrons irrespective of their structure or genetic context. We identified several examples of class 1 integrons linked to complete Tn402-like or Tn402 hybrid transposition modules and diverse insertion points with respect to the inverted repeat IRi boundary. The diversity and abundance of class 1 integrons identified are such that many novel elements seen here would not have been identified by commonly used methods, and they revealed an additional level of complexity.
More Related Videos
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
08:03Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
Related Concept Videos
Introduction to the Human Microbiota
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies. Common...
Evolution of Microbial Genome
Antibiotic Selection
Determinants of Bacterial Pathogenicity and Virulence