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Related Experiment Videos

Platelet-activating factor stimulates angiotensin converting enzyme activity.

H Kawaguchi1, H Sawa, K Iizuka

  • 1Department of Cardiovascular Medicine, Hokkaido University School of Medicine, Sapporo, Japan.

Journal of Hypertension
|February 1, 1990
PubMed
Summary

Platelet-activating factor (PAF) enhances angiotensin I to angiotensin II conversion in lung cells. This suggests PAF plays a role in regulating vascular tone by modulating angiotensin converting enzyme (ACE) activity.

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Area of Science:

  • Cardiovascular Physiology
  • Endothelial Cell Biology
  • Enzyme Kinetics

Background:

  • Vascular tone is regulated by the renin-angiotensin system.
  • Pulmonary artery endothelial cells play a role in cardiovascular regulation.
  • Platelet-activating factor (PAF) is a lipid mediator with diverse biological effects.

Purpose of the Study:

  • To investigate the effect of PAF on angiotensin converting enzyme (ACE) activity in pulmonary artery endothelial cells.
  • To determine the kinetic parameters of ACE in the presence and absence of PAF.
  • To explore the potential role of PAF in modulating vascular tone via ACE activity.

Main Methods:

  • Primary culture of pulmonary artery endothelial cells.
  • Measurement of angiotensin I to angiotensin II conversion.

Related Experiment Videos

  • Enzyme kinetic analysis (Vmax and Km determination).
  • Pharmacological inhibition using ACE inhibitors (enalapril) and a PAF antagonist (CV3988).

    • Main Results:

    • PAF significantly enhanced the conversion of angiotensin I to angiotensin II in a dose-dependent manner.
    • The apparent Vmax and Km for ACE were determined to be 2.5 nmol/min per dish and 50 µmol/l, respectively.
    • PAF-induced enhancement of ACE activity was suppressed by enalapril and CV3988.
    • Bradykinin inhibited ACE activity, while other phospholipids did not.

    Conclusions:

    • PAF stimulates angiotensin converting enzyme (ACE) activity in pulmonary artery endothelial cells.
    • This PAF-mediated enhancement of ACE activity suggests a novel mechanism for regulating vascular tone.
    • PAF may be an important modulator of the renin-angiotensin system in the pulmonary vasculature.