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Updated: Jun 1, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing
Wasun Chantratita1, Chonlaphat Sukasem, Sayomporn Sirinavin
1Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok. rawct@mahidol.ac.th
Introduction:
We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.
Methodology:
This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing.
Results:
From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing.
Conclusions:
The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.
Insights
A swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand showed a low frequency of the Oseltamivir resistance mutation H274Y. One case of natural variation conferring resistance was identified in a patient not previously treated with anti-influenza drugs.
Area of Science:
- Virology
- Antimicrobial Resistance
Background:
- Investigating the prevalence of the H274Y mutation in swine-origin 2009 A (H1N1) influenza virus in Thailand.
- Focusing on the period of May-August 2009 during a significant influenza outbreak.
Observation:
- A total of 8,710 real-time RT-PCR positive specimens for swine-origin 2009 A (H1N1) influenza virus were analyzed.
- Pyrosequencing was employed to detect the Oseltamivir resistance mutation H274Y.
- One out of 100 randomly selected samples exhibited the H274Y mutation.
Findings:
- The H274Y mutation, conferring Oseltamivir resistance, was found at a low frequency in the studied population.
- The identified case likely acquired resistance through natural variation, as the patient had no prior exposure to Oseltamivir.
Implications:
- Highlights the potential for spontaneous emergence of antiviral resistance in influenza viruses.
- Underscores the importance of continuous surveillance for antiviral resistance mutations in circulating influenza strains.
- Informs treatment strategies and public health responses during influenza outbreaks.
