Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation.

Yi Cheng¹, Xiaolong Luo, Chen-Yu Tsao

¹Institute for Systems Research (ISR), University of Maryland, College Park, MD 20742, USA.

|June 2, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of a magnetic field on the activity of superoxide dismutase studied at the enzyme level.

RSC advances·2026

Same author

Mediated Electrochemical Probing and Machine Learning for Cysteine and Reduced Monoclonal Antibody Quantification.

Biotechnology and bioengineering·2026

Same author

Electrochemical Phase Engineering of γ'‑V<sub>2</sub>O<sub>5</sub> Thin Films for Sodium-Ion Storage Electrodes.

ACS omega·2026

Same author

Sustainable agent of compost-derived bacteria for enhanced biological hydrolysis and saccharification of Napier grass.

Bioresource technology·2025

Same author

Scrambling Signal Modularity in Bottom-up Assembled Synthetic Pseudomonas Consortia Reveals Robust Information Transfer.

ACS synthetic biology·2025

Same author

Electrochemistry as a Tool for Redox-Based Bio-Information Processing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Researchers developed a new method for 3D cell assembly using stimuli-responsive alginate gels in microfluidic devices. This technique allows for electrically triggered, programmable cell organization and in vitro study of cellular processes.

Area of Science:

Biomaterials engineering
Cellular biology
Microfluidics

Background:

Developing methods for precise 3D cell assembly is crucial for studying cellular interactions.
Stimuli-responsive materials offer potential for controlled biological manipulation.

Purpose of the Study:

To demonstrate programmable 3D cell assembly under physiological conditions using electrodeposited alginate gels.
To enable in vitro studies of dynamic cellular and intercellular processes.

Main Methods:

Utilized electrodeposited stimuli-responsive alginate gels within a microfluidic device.
Employed parallel sidewall electrodes for electrical triggering and direct observation of cell assembly.
Tested assembly of mammalian cells and different strains of E. coli.

More Related Videos

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Published on: December 21, 2019

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Related Experiment Videos

Last Updated: Jun 1, 2026

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Published on: March 21, 2019

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Published on: December 21, 2019

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Main Results:

Achieved programmable 3D cell assembly at physiological pH.
Demonstrated electrically triggered assembly and maintained viability of mammalian cells.
Showcased spatially programmable, multi-address assembly of different E. coli strains.

Conclusions:

The developed microfluidic system enables precise control over cell assembly using electrical stimuli.
This platform facilitates in vitro investigation of complex cellular behaviors, including signaling and growth.
The approach holds promise for advanced cell-based assays and tissue engineering applications.