Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalised Pathways (PP) in the Medical Curriculum: How Does It Influence Students?

The clinical teacher·2026
Same author

Cantilever-based optical interfacial force microscope in liquid using photothermal actuation.

The Review of scientific instruments·2025
Same author

The effectiveness of basivertebral nerve radiofrequency ablation for the treatment of vertebrogenic low back pain: 1-year results of a prospective real-world cohort study.

Pain medicine (Malden, Mass.)·2025
Same author

Translating faculty development into practice and professional identity: The lived experience of clinical educators.

Medical teacher·2025
Same author

The effect of lumbar medial branch radiofrequency neurotomy on cobb angle progression in individuals with adult scoliosis compared to natural history: A cross-sectional study.

Interventional pain medicine·2024
Same author

Does medial branch radiofrequency neurotomy accelerate degenerative lumbar spondylolisthesis compared to natural progression? A cross-sectional cohort study.

Interventional pain medicine·2024

Related Experiment Video

Updated: Jun 1, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Long-term structural changes of plasmid DNA studied by atomic force microscopy.

Byung I Kim1, Reilly Clark, Tyler Clark

  • 1Department of Physics, Boise State University, Boise, Idaho, USA. ByungKim@boisestate.edu

Scanning
|June 2, 2011
PubMed
Summary

Plasmid DNA (pDNA) structures change over four years from supercoiled forms to branched networks. Residual proteins likely drive these long-term structural transformations in pDNA solutions.

More Related Videos

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Related Experiment Videos

Last Updated: Jun 1, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Area of Science:

  • Molecular Biology
  • Biophysics
  • Biochemistry

Background:

  • Long-term stability of plasmid DNA (pDNA) conformations is crucial for research, particularly gene therapy applications.
  • Understanding the time-evolution of pDNA structures at a molecular level is essential but has been understudied.

Purpose of the Study:

  • To investigate the structural changes of plasmid DNA (pDNA) over a four-year period.
  • To elucidate the molecular mechanisms underlying pDNA structural transformations.

Main Methods:

  • Atomic Force Microscopy (AFM) was employed to observe pDNA solutions over four years.
  • Topographical analysis of AFM data was conducted to assess structural details.

Main Results:

  • pDNA structures evolved from isolated supercoiled forms to aggregated supercoiled structures.
  • Further evolution led to the formation of thin, branched network structures, and subsequently wider branched networks.
  • Topographical analysis indicated residual proteins as a potential key factor in these observed structural changes.

Conclusions:

  • Plasmid DNA (pDNA) undergoes significant structural modifications over extended periods.
  • Residual proteins are suggested as the primary drivers of these long-term structural changes in laboratory-prepared pDNA.
  • These findings have implications for the stability and application of pDNA in fields like gene therapy.