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Membrane filter enumeration method for Clostridium perfringens.

J W Bisson, V J Cabelli

    Applied and Environmental Microbiology
    |January 1, 1979
    PubMed
    Summary
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    This study introduces a rapid membrane filter method for detecting Clostridium perfringens in water. The new technique offers accurate quantification and faster results compared to traditional methods.

    Area of Science:

    • Environmental microbiology
    • Water quality assessment
    • Bacteriology

    Background:

    • Clostridium perfringens is an indicator of fecal contamination in aquatic environments.
    • Accurate and rapid detection methods are crucial for public health and environmental monitoring.
    • Existing methods can be time-consuming and less efficient.

    Purpose of the Study:

    • To develop and validate a rapid membrane filter procedure for quantifying Clostridium perfringens.
    • To improve detection accuracy and reduce enumeration time for water samples.
    • To provide a reliable alternative to existing methods like the Bonde pour tube method.

    Main Methods:

    • Utilized a selective medium containing D-cycloserine, polymyxin B sulfate, and specific differential characteristics (sucrose fermentation, acid phosphatase production, absence of beta-D-glucosidase).

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  • Incubation at 45°C to inhibit background growth.
  • Enumeration of colonies on membrane filters within 18-24 hours.

    • Main Results:

    • Achieved 93% verification of typical colonies.
    • Reported average recovery rates of 79% from spore suspensions and 90% from seawater suspensions.
    • Demonstrated precision comparable to random error and superior recovery compared to the Bonde pour tube method.

    Conclusions:

    • The developed membrane filter procedure provides a rapid, accurate, and reliable method for Clostridium perfringens quantitation in aquatic environments.
    • This method enhances water quality monitoring capabilities.
    • Offers a significant improvement over conventional techniques for detecting this bacterium.