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Updated: Jun 1, 2026
Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice
Published on: May 10, 2019
Age-related hearing loss and expression of antioxidant enzymes in BDF1 mice
Keiji Tabuchi1, Tomofumi Hoshino, Yuki Hirose
1Department of Otolaryngology, Head and Neck Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan. ktabuchi@md.tsukuba.ac.jp
Conclusion:
Our data suggest that the changes in expression of antioxidant enzymes may cause age-related hearing loss (AHL).
Objectives:
AHL is an aging process of the inner ear, and oxidant stressors are considered to be one of the leading causes. We investigated the hearing level and expression profile of antioxidant enzymes in aged mice.
Methods:
Mice aged 3, 6, and 11 months were used. Hearing levels of the mice were examined using the auditory brainstem response (ABR). After measuring the ABR threshold, cochleae were dissected. RNA was isolated from the cochleae, and cDNA was synthesized using the retro-transcription enzyme. Expression of the antioxidant enzymes was measured by quantitative real-time PCR.
Results:
The ABR thresholds of the BDF1 mice were elevated by 6 months of age. The expression of superoxide dismutase 1 (SOD1) and heme oxygenase 1 (HO1) at 11 months of age significantly decreased compared with that of those at 6 months of age. In contrast, a decrease in the expression level was not observed regarding NAD(P)H-quinone oxidoreductase 1 (NQO1).
