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Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...

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Related Experiment Video

Updated: Jun 1, 2026

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

Effect of geometric challenges on cell migration.

Richard J Mills1, Jessica E Frith, James E Hudson

  • 1Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Australia.

Tissue Engineering. Part C, Methods
|June 3, 2011
PubMed
Summary
This summary is machine-generated.

Cell migration in 3D scaffolds depends on architecture. A microfluidic device revealed NIH 3T3 fibroblasts and human mesenchymal stromal cells (hMSCs) respond differently to geometric cues, linked to N-cadherin expression.

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Related Experiment Videos

Last Updated: Jun 1, 2026

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Cellular infiltration into 3D porous scaffolds is crucial for tissue engineering.
  • Scaffold internal architecture significantly influences cell colonization and migration.
  • Understanding cell-environment interactions is key to designing effective tissue constructs.

Purpose of the Study:

  • To develop and validate a microfluidic device simulating 3D scaffold architecture in 2D.
  • To investigate how geometric constraints affect NIH 3T3 fibroblast and human mesenchymal stromal cell (hMSC) migration.
  • To elucidate the role of N-cadherin in differential cell migration responses.

Main Methods:

  • Development of a microfluidic device with controlled geometric features (channel width, tortuosity, contractions, expansions, junctions).
  • Observation of NIH 3T3 fibroblast and hMSC migration patterns within the microfluidic device.
  • Analysis of N-cadherin expression and functional inhibition in relation to cell migration.

Main Results:

  • NIH 3T3 fibroblasts migrated collectively, while hMSCs migrated individually.
  • Fibroblast migration was sensitive to various geometric constraints, unlike hMSCs, which were mainly affected by channel width.
  • Higher N-cadherin expression in fibroblasts correlated with their distinct migration behavior; inhibition mimicked hMSC migration.

Conclusions:

  • The microfluidic device effectively mimics 3D scaffold architectural challenges.
  • Cellular migration characteristics, influenced by factors like N-cadherin, dictate responses to geometric constraints.
  • Findings provide insights into cell behavior in porous constructs for tissue engineering applications.