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Updated: Jun 1, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Published on: June 12, 2017
MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II
Jian Xiao1, Xiaoyan Zhu, Bin He
1Department of Cardiothoracic Surgery, Changzheng Hospital, the Second Military Medical University, Fengyang road #415, Shanghai, China.
Background:
Autophagy plays a significant role in myocardial ischemia-reperfusion (IR) injury. So it is important to inhibit autophagy to protect cardiomyocytes besides anti-apoptosis. MiRNA has been demonstrated to protect cardiomyocytes against apoptosis during IR, while whether it has anti-autophagy effect has not been known. The aim of this study was to investigate whether miR-204 regulated autophagy by regulating LC3-II protein, which is the marker of autophagosome during myocardial IR injury.
Methods:
Adult SD rats were randomized to Control and IR groups. IR group was treated with 30 min ischemia by ligating the left anterior descending coronary artery, followed by 2 h reperfusion by loosing the ligation. The expression of miR-204 was measured by RT-PCR, and LC3 protein was measured by western-blot.
Results:
We found that IR induced cardiomyocytes autophagy, together with down-regulation of miR-204 and up-regulation of LC3-II protein. And, we have found that LC3-II protein was regulated by miR-204, using the method of transferring miR-204 mimic or AMO-204 into the cardiomyocytes, before.
Conclusions:
These studies provided evidence that miR-204 played an important role in regulating autophagy through LC3-IIprotein during IR.
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