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Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness
E Miko1, M Halasz, B Jericevic-Mulac
1Department of Medical Microbiology and Immunology, Medical School, Pecs University, 12 Szigeti Street, H-7624 Pecs, Hungary.
Journal of Reproductive Immunology
|June 3, 2011
Summary
Progesterone-induced blocking factor (PIBF) regulates trophoblast invasion. PIBF expression inversely correlates with invasiveness, suggesting PIBF affects leptin signaling in pregnancy and tumors.
Area of Science:
- Reproductive Biology
- Molecular Endocrinology
- Cancer Biology
Background:
- Controlled trophoblast invasion is crucial for successful human pregnancy.
- Progesterone and its induced molecule, PIBF, regulate trophoblast invasiveness.
- Placental leptin also influences trophoblast invasion by affecting progesterone secretion.
Purpose of the Study:
- To investigate the interaction between PIBF and leptin in regulating trophoblast invasion.
- To analyze PIBF and leptin/leptin receptor expression in normal and abnormal placentae.
- To determine PIBF's effect on leptin and leptin receptor expression in trophoblast cells.
Main Methods:
- Immunohistochemistry for PIBF, leptin, and leptin receptor on placental tissues.
- Generation of PIBF-deficient trophoblast cells using siRNA.
- Western blot analysis for leptin receptor and protein array for leptin expression.
Main Results:
- PIBF expression was high in normal and partial mole placentae, decreased in complete mole, and absent in choriocarcinoma.
- Leptin and leptin receptor were absent in partial mole but present in complete mole and choriocarcinoma.
- PIBF deficiency upregulated leptin receptor, while PIBF treatment decreased leptin expression.
Conclusions:
- PIBF expression is inversely related to trophoblast invasiveness.
- PIBF influences the expression of leptin and its receptor.
- This interaction may play a role in placentation and tumor development.
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