Related Experiment Videos
[Production and characterization of hybrid monoclonal antibodies with IgG1/IgG3 double isotype]
V Lebègue1, L Joron, J P Barque
1D.I.C.A. Division d'Immuno-Cytologie appliquée, Université de Technologie de Compiègne.
In order to obtain bispecific monoclonal antibodies, two hybridoma cell lines were fused: E13 producing an IgG1 anti-Cytomegalovirus, and 70B12 producing an IgG3 anti-Chlamydia. This experimental model was chosen to test the possibility of association between gamma 1 gamma 3 heavy chains. This association was shown by using an ELISA.
In order to obtain bispecific monoclonal antibodies, two hybridoma cell lines were fused: E13 producing an IgG1 anti-Cytomegalovirus, and 70B12 producing an IgG3 anti-Chlamydia. This experimental model was chosen to test the possibility of association between gamma 1 gamma 3 heavy chains. This association was shown by using an ELISA.