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Related Experiment Video

Updated: Jun 1, 2026

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
14:53

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

Published on: May 19, 2011

High-throughput screening and biosensing with fluorescent C. elegans strains.

Chi K Leung1, Andrew Deonarine, Kevin Strange

  • 1Department of Biology, University of Florida, FL, USA.

Journal of Visualized Experiments : Jove
|June 3, 2011
PubMed
Summary

This study presents a high-throughput screening protocol using Caenorhabditis elegans (C. elegans) for drug discovery and environmental monitoring. The method efficiently screens chemical libraries and detects contaminants by measuring gene expression changes in vivo.

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Area of Science:

  • Pharmacology and Toxicology
  • Genetics and Molecular Biology
  • Environmental Science

Background:

  • High-throughput screening (HTS) using cell cultures often yields compounds toxic or inactive in vivo.
  • Whole animal models streamline drug development by avoiding in vitro limitations.
  • Caenorhabditis elegans (C. elegans) is a tractable, cost-effective model organism suitable for HTS.

Purpose of the Study:

  • To describe a protocol for culturing and dispensing fluorescent C. elegans strains for HTS.
  • To enable screening of chemical libraries for biological modulators.
  • To develop biosensor assays for detecting environmental contaminants via gene expression.

Main Methods:

  • Developmentally synchronized C. elegans are cultured in liquid, harvested, and suspended at a defined density.

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A High-throughput, High-content, Liquid-based C. elegans Pathosystem
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Last Updated: Jun 1, 2026

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
14:53

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

Published on: May 19, 2011

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health
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High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

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A High-throughput, High-content, Liquid-based C. elegans Pathosystem
09:44

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

Published on: July 1, 2018

  • Worms are dispensed into 384-well plates using a peristaltic liquid dispenser.
  • Real-time fluorescence intensity is measured in vivo using a microplate reader after compound or sample addition.

    • Main Results:

    • The protocol is adaptable to any inducible gene in C. elegans with a suitable reporter strain.
    • Demonstrated HTS assay monitors the transcription factor SKN-1 using a gst-4::GFP reporter.
    • The gst-4 reporter serves as a biosensor for xenobiotic and oxidative chemicals, detecting contaminants like acrylamide and methyl-mercury.

    Conclusions:

    • This C. elegans-based HTS protocol offers a robust method for drug discovery and environmental contaminant detection.
    • The system leverages transgenic reporter strains to monitor specific biological pathways in vivo.
    • The SKN-1/gst-4 pathway assay provides a sensitive tool for assessing chemical toxicity and environmental pollutants.