Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Site-specific transposition of insertion sequence IS630.

T Tenzen1, S Matsutani, E Ohtsubo

  • 1Institute of Applied Microbiology, University of Tokyo, Japan.

Journal of Bacteriology
|July 1, 1990
PubMed
Summary

IS630, a mobile genetic element in Shigella sonnei, was studied for its transposition activity. Researchers found IS630 preferentially inserts into TA sequences, suggesting a specific mechanism for its movement within bacterial genomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Addition formulae for Abelian functions associated with specialized curves.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2011
Same author

Trajectory and terminal distribution of single centrifugal axons from olfactory cortical areas in the rat olfactory bulb.

Neuroscience·2010
Same author

Therapeutic effect of balloon-occluded retrograde transvenous obliteration for gastric varices in relation to haemodynamics in the short gastric vein.

The British journal of radiology·2009
Same author

Application of percutaneous ultrasound-guided treatment for ultrasonically invisible hypervascular hepatocellular carcinoma using microbubble contrast agent.

Clinical radiology·2007
Same author

Tonsillectomy in the treatment of pediatric Henoch-Schönlein nephritis.

Clinical nephrology·2007
Same author

Real-time blood-pool images of contrast enhanced ultrasound with Definity in the detection of tumour nodules in the liver.

The British journal of radiology·2005

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • IS630 is a mobile genetic element identified in Shigella sonnei.
  • Unlike many mobile elements, IS630 does not appear to mediate cointegration between different replicons.
  • Understanding the transposition mechanism of IS630 is crucial for comprehending bacterial genome dynamics and evolution.

Purpose of the Study:

  • To investigate the transposition activity and insertion specificity of the IS630 element.
  • To determine the preferred target sequences for IS630 transposition.
  • To elucidate the role of flanking sequences in IS630's transposition mechanism.

Main Methods:

  • Construction of composite elements with inverted copies of IS630 flanking a drug resistance gene.
  • Introduction of these composite elements into Escherichia coli containing plasmid ColE1.
  • DNA sequencing to identify transposition insertion sites and analyze flanking sequences.
  • In vitro mutagenesis to assess the impact of nucleotide substitutions at the IS630 ends on transposition activity.

Main Results:

  • Composite elements containing IS630 demonstrated transposition to plasmid ColE1 in Escherichia coli.
  • Transposition exclusively occurred at the dinucleotide sequence 5'-TA-3'.
  • Two preferred insertion sites were identified within the TA sequences of a 13-base-pair stem region of a rho-dependent transcription terminator.
  • Mutagenesis of the TA sequences at the IS630 ends did not affect transposition activity or target site preference.

Conclusions:

  • IS630 exhibits specific transposition activity, favoring insertion into TA dinucleotides.
  • The 13-base-pair inverted repeat region, particularly the TA sequences within it, plays a role in directing IS630 insertion.
  • IS630 transposition mechanism involves target duplication of the TA sequence, independent of its own flanking nucleotides.

Related Experiment Videos