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Updated: Jul 14, 2026
13:04
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Cultivation of hepatitis A virus in various cell lines and isolation of DNA sequences]
Voprosy Virusologii
|January 1, 1990
Abstract:
Using the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.
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