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Related Experiment Video

Updated: Jun 1, 2026

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

Plk1 is negatively regulated by RNF8.

Takashi Yoshioka¹, Masashi Kimura, Masanao Saio

¹Department of Molecular Pathobiochemistry, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.

Biochemical and Biophysical Research Communications

|June 4, 2011

Summary

RNF8 protein depletion inhibits cell growth and causes cell death. This nuclear protein is downregulated in cancer cells, inversely correlating with increased Plk1 levels.

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

RNF8 (RING finger protein 8) is a nuclear protein with FHA and RF domains.
RNF8 plays a role in cell cycle regulation and integrity.

Purpose of the Study:

To investigate the function of RNF8 in the M phase of the cell cycle.
To explore the relationship between RNF8 and Plk1 (Polo-like kinase 1).

Main Methods:

RNF8 depletion using siRNA.
Cell cycle analysis and time-lapse microscopy.
Western blotting to assess protein levels of RNF8 and Plk1 in various cell lines.

Main Results:

RNF8 depletion led to cell growth inhibition, cell cycle arrest (S and G2/M phases), and cell death.
RNF8-depleted cells exhibited increased Plk1 levels.
RNF8 expression was significantly lower in cancer cell lines compared to normal cell lines, while Plk1 expression was higher.

Conclusions:

RNF8 is downregulated in many cancer cells.
RNF8 downregulation is inversely correlated with Plk1 levels.
RNF8 may function as a tumor suppressor by regulating cell cycle progression and Plk1 activity.