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Related Experiment Video

Updated: Jun 1, 2026

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Reversible thrombin detection by aptamer functionalized STING sensors.

Paolo Actis1, Adam Rogers, Jeff Nivala

  • 1Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.

Biosensors & Bioelectronics
|June 4, 2011
PubMed
Summary
This summary is machine-generated.

Signal Transduction by Ion NanoGating (STING) sensors offer label-free detection. Aptamer-functionalized STING biosensors enable quantitative thrombin detection through correlated ionic current signals.

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Area of Science:

  • Biosensing technologies
  • Nanotechnology
  • Electrochemistry

Background:

  • Signal Transduction by Ion NanoGating (STING) is a label-free biosensing technology utilizing functionalized quartz nanopipettes.
  • STING sensors are fabricated from inexpensive quartz capillaries and employ an electrochemical system for signal transduction.
  • Previous limitations in STING technology included difficulties in correlating ionic current to analyte concentration.

Purpose of the Study:

  • To demonstrate the quantitative detection of thrombin using aptamer-functionalized STING sensors.
  • To establish a direct correlation between measured ionic current and thrombin concentration.

Main Methods:

  • Fabrication of STING sensors using quartz nanopipettes.
  • Functionalization of nanopipette pores with aptamers as recognition elements.
  • Utilizing an electrochemical system to transduce molecular binding events.
  • Correlating changes in ionic current to the concentration of bound thrombin.

Main Results:

  • STING sensors functionalized with aptamers successfully detected thrombin.
  • A direct correlation was established between the generated signal and thrombin concentration.
  • The developed biosensing platform allows for quantitative analysis of thrombin in solution.

Conclusions:

  • Aptamer-functionalized STING sensors provide a viable method for quantitative thrombin detection.
  • This advancement overcomes previous limitations in correlating sensor signals to analyte concentration.
  • The STING platform demonstrates potential for sensitive and label-free biosensing applications.