Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Epitope-Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi-Epitope Bispecific Antibody Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026

Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026

Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026

Same author

Nanobiosensors and Artificial Intelligence Strategies for Glycan Profiling in Cancer Progression: A Critical Review.

ACS sensors·2026

Same author

Magnetoelectrically Enhanced Molecular Recognition on Plasmonic Surfaces.

Small (Weinheim an der Bergstrasse, Germany)·2026

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

Rapid DNA detection by beacon-assisted detection amplification.

Ashley R Connolly¹, Matt Trau

¹Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane, Queensland, Australia. a.connolly@uq.edu.au

Nature Protocols

|June 4, 2011

Summary

This study introduces a rapid DNA amplification and detection method using a DNA nanomachine. The novel isothermal process quickly identifies specific DNA sequences in biological samples within 40 minutes.

More Related Videos

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 1, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Accurate DNA detection is crucial for diagnostics and research.
Existing methods for DNA amplification and detection can be time-consuming and complex.
There is a need for rapid, sensitive, and user-friendly DNA analysis techniques.

Purpose of the Study:

To develop and present a novel, rapid isothermal protocol for DNA amplification and detection.
To utilize a DNA nanomachine with dual molecular switches for enhanced specificity and efficiency.
To enable the detection of femtomole amounts of specific DNA sequences in under 40 minutes.

Main Methods:

A DNA nanomachine comprising two molecular switches (detection and amplification/signal-transduction) was designed.
The protocol employs a molecular beacon detection switch activated by the target DNA sequence.
Isothermal amplification is achieved using a nicking endonuclease and DNA polymerase at 40 °C in a single reaction.

Main Results:

The DNA nanomachine successfully amplified and detected specific DNA targets isothermally.
The protocol demonstrated high sensitivity, enabling detection of femtomole amounts of DNA.
The entire process, from amplification to detection, was completed in less than 40 minutes.
The method proved effective with both synthetic DNA and purified DNA from biological samples.

Conclusions:

This novel isothermal protocol offers a rapid and efficient method for DNA amplification and detection.
The DNA nanomachine design provides a sensitive and specific platform for molecular diagnostics.
The protocol's speed and simplicity make it suitable for various applications, including clinical settings.