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The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

Mouse corneal lymphangiogenesis model.

Renhai Cao¹, Sharon Lim, Hong Ji

¹Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Nature Protocols

|June 4, 2011

Summary

This summary is machine-generated.

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This study presents a novel in vivo method to analyze new lymphatic vessel formation in the mouse cornea. This technique allows for quantitative assessment of lymphangiogenesis without interference from existing lymphatic networks.

Area of Science:

Ophthalmology
Angiogenesis Research
Vascular Biology

Background:

Studying lymphatic vessel formation (lymphangiogenesis) in avascular tissues like the cornea is challenging due to the absence of pre-existing lymphatics.
Existing methods may be confounded by endogenous lymphatic networks, complicating the analysis of induced neolymphangiogenesis.

Purpose of the Study:

To describe a robust in vivo protocol for quantitatively studying lymphangiogenesis in the avascular mouse cornea.
To provide a method that circumvents interference from pre-existing lymphatic vessels for accurate neolymphangiogenesis assessment.

Main Methods:

Surgically creating a micropocket in the mouse cornea and implanting slow-release polymers loaded with lymphangiogenic factors (e.g., VEGF-A, VEGF-C, FGF-2).
Detecting newly formed lymphatic vessels using immunohistochemical staining with specific markers like LYVE-1 and VEGFR-3 on flattened corneal tissue.

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Last Updated: Jun 1, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

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Published on: August 16, 2014

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A Mouse Model for Laser-induced Choroidal Neovascularization

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Published on: December 27, 2015

Quantitatively analyzing lymphatic vessel growth and its relationship with hemangiogenesis.

Main Results:

Successful induction of a robust lymphangiogenic response in the mouse cornea following factor implantation.
Detection of new lymphatic vessel growth starting around day 5-6 post-implantation, persisting for approximately 14 days.
Demonstration of the method's ability to visualize and quantify lymphatic vessel development in relation to blood vessel formation.

Conclusions:

This protocol offers a powerful and quantitative in vivo model for studying lymphangiogenesis in an avascular environment.
The method facilitates research into the mechanisms of lymphatic vessel formation, remodeling, and function.
It provides a valuable tool for investigating therapeutic strategies targeting lymphangiogenesis in ocular diseases.