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Updated: Jun 1, 2026
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
Published on: October 20, 2018
Three-dimensional polycaprolactone hierarchical scaffolds supplemented with natural biomaterials to enhance
Hyeon Yoon1, Seunghyun Ahn, Geunhyung Kim
1Bio/Nanofluidics Lab, Department of Mechanical Engineering, Chosun University, 375 Seosok-dong, Gwangju 501-759, Korea.
Abstract:
A hybrid technology that combines a three-dimensional (3-D) dispensing system with an electrospinning process was used to produce a hierarchical 3-D scaffold consisting of micro-sized polycaprolactone (PCL) strands and micro/nano-sized fibres. The micro/nanofibre biocomposites electrospun with PCL/small intestine submucosa (SIS) and PCL/Silk fibroin were layered between melt-plotted micro-strands. The scaffold containing SIS exhibited a stronger hydrophilic property than other scaffolds due to the various hydrophilic components in SIS. The 3-D hierarchical scaffold having biocomposites exhibited an incredibly enhanced initial cell attachment and proliferation of bone marrow-derived mesenchymal stem cells relative to the normally designed 3-D scaffold.
