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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Intrageneric primer design: Bringing bioinformatics tools to the class.

André O S Lima1, Sérgio P S Garcês

  • 1Centro de Ciências Tecnológicas da Terra e do Mar, Universidade do Vale do Itajaí, R. Uruguai, 458, Itajaí-SC 88302-202, Brazil. andreolima@hotmail.com.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
|June 4, 2011
PubMed
Summary

This study introduces a problem-based bioinformatics class for undergraduates to design PCR primers. Students learn essential bioinformatics procedures using freeware tools and online databases.

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Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomic Analysis

Background:

  • Bioinformatics is a rapidly expanding scientific field utilizing informatics for biological data analysis.
  • There's a growing demand for professionals with bioinformatics skills in both academic and private sectors.
  • Existing educational approaches may not adequately prepare bachelor of science students for these demands.

Purpose of the Study:

  • To describe a problem-based learning module for undergraduate bioinformatics education.
  • To equip students with practical skills in designing intrageneric primers for Polymerase Chain Reaction (PCR).
  • To familiarize students with key bioinformatics procedures and tools.

Main Methods:

  • A five-session, 1-hour per session, problem-based class structure.
  • Utilized freely available bioinformatics software and internet-based databases.
  • Focused on practical application through primer design for PCR.

Main Results:

  • Students successfully designed intrageneric primers for PCR.
  • Students gained hands-on experience with major bioinformatics procedures.
  • Demonstrated the effectiveness of problem-based learning in bioinformatics education.

Conclusions:

  • The described problem-based class effectively teaches essential bioinformatics skills.
  • This approach addresses the need for skilled bioinformatics professionals.
  • Students learn practical applications of database searching, sequence alignment, and primer selection.