Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lipid transfer proteins and PI4KIIα generate a phosphoinositide-linked proteome.

The Journal of biological chemistry·2026

Same author

Development and characterization of an inducible Tensin1 deficient transgenic murine model.

Scientific reports·2026

Same author

Lipid Transfer Proteins and PI4KIIα Generate a Phosphoinositide-Linked Proteome.

bioRxiv : the preprint server for biology·2025

Same author

Toward a common standard for data and specimen provenance in life sciences.

Learning health systems·2024

Same author

Kinematic changes in gait in boys with Duchenne Muscular Dystrophy: Utility of the Gait Deviation Index, the Gait Profile Score and the Gait Variable Scores.

Gait & posture·2022

Same author

Differential regulation of G protein signaling in <i>Arabidopsis</i> through two distinct pathways that internalize AtRGS1.

Science signaling·2021

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Microarrays (DNA chips) for the classroom laboratory.

Betsy Barnard¹, Michael Sussman, Sandra Splinter Bondurant

¹West High School, Madison, Wisconsin 53726. bbarnard@wisc.edu.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology

|June 4, 2011

Summary

High school students can now perform DNA microarray experiments affordably. This accessible technology uses a printable DNA chip and simulated targets to study gene expression in organisms like Arabidopsis thaliana.

More Related Videos

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Related Experiment Videos

Last Updated: Jun 1, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Area of Science:

Molecular Biology
Genetics
Biotechnology Education

Background:

DNA microarray technology is a powerful tool for studying gene expression.
Traditional microarray methods are often costly and complex for high school settings.
There is a need for accessible, hands-on biotechnology tools for secondary education.

Purpose of the Study:

To develop and optimize low-cost, user-friendly DNA microarray materials for high school students.
To adapt existing research tools for classroom use in molecular biology.
To demonstrate differential gene expression analysis using accessible technology.

Main Methods:

Students manually create a low-density DNA chip/array.
A simulated cDNA target is used for analysis.
Adapted research tools are employed for gene expression analysis.
Arabidopsis thaliana genes related to photosynthesis were studied.

Main Results:

Successfully demonstrated differential gene expression in key plant genes.
Developed a cost-effective and simplified DNA microarray protocol.
Validated the method's applicability to various biological organisms and experimental conditions.

Conclusions:

This accessible DNA microarray technology empowers high school students with practical molecular biology skills.
The developed methods provide a foundation for diverse gene expression studies in educational settings.
The approach has been successfully implemented and shared with educators.