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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 1, 2026

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Microarrays for undergraduate classes.

Dale Hancock¹, Lisa L Nguyen, Gareth S Denyer

¹University of Sydney, New South Wales, Australia 2006. D.Hancock@mmb.usyd.edu.au.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology

|June 4, 2011

Summary

This summary is machine-generated.

Undergraduate students can now perform complex gene expression analysis using microarray technology. This hands-on experiment covers RNA isolation to data analysis, offering valuable research experience.

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Last Updated: Jun 1, 2026

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

Molecular Biology
Genomics
Biotechnology Education

Background:

Murine erythroleukemia cells offer a scalable model for studying gene expression changes.
Erythropoietic agents like DMSO induce significant gene expression alterations within 72 hours.
Gene expression analysis is crucial for understanding cellular differentiation and disease.

Purpose of the Study:

To provide undergraduate students with practical experience in microarray-based gene expression analysis.
To integrate a comprehensive molecular biology workflow, from sample preparation to data interpretation, into a laboratory course.
To familiarize students with cutting-edge techniques used in modern research settings.

Main Methods:

Isolation of total RNA from control and DMSO-treated murine erythroleukemia cells.
Synthesis of complementary DNA (cDNA) incorporating amino-allyl dUTP and labeling with Cy3/Cy5 dyes.
Hybridization of labeled cDNA to a standard cDNA microarray, followed by washing, scanning, and data analysis using a dedicated program.

Main Results:

Successful induction of large gene expression changes in murine erythroleukemia cells by DMSO.
Identification of up- and down-regulated genes through microarray analysis.
Validation of array data using internal controls based on well-characterized erythroid sequences.

Conclusions:

The presented microarray experiment effectively trains undergraduate students in advanced molecular biology techniques and data analysis.
Incorporating quality control measures and internal controls enhances the reliability of the experimental results.
This curriculum provides students with invaluable hands-on experience with research-grade technology, bridging the gap between academic learning and scientific discovery.