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Published on: February 1, 2018
LC-Biosensor System for the Determination of the Neurotoxin β-N-Oxalyl-l-α,β-diaminopropionic Acid
1Department of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia, Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania, and Department of Analytical Chemistry, University of Lund, P.O. Box 124, S-221 00, Lund, Sweden.
Abstract:
An analysis system is described for the determination of the neurotoxin β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP). The system is based on liquid chromatographic separation of β-ODAP from interfering amino acids on an anion exchange column coupled with an amperometric enzyme electrode for the registration of β-ODAP. The electrode is based on a graphite rod modified with an Os(2+/3+) redox polymer cross-linked with l-glutamate oxidase and horseradish peroxidase. This LC-bienzyme electrode analytical system enabled monitoring of as low as 4 μM β-ODAP (injection volume 100 μL). The β-ODAP content in real grass pea samples was measured to range between 3.3 and 5.2 g kg(-)(1) in dry grass pea.
