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Updated: Jun 1, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

A Microscale NO(3)(-) Biosensor for Environmental Applications.

L H Larsen¹, T Kjær, N P Revsbech

¹Department of Microbial Ecology, Institute of Biological Sciences, University of Aarhus, Bd. 540, DK-8000 Aarhus C, Denmark.

Analytical Chemistry

|June 7, 2011

Summary

This summary is machine-generated.

A novel biosensor effectively measures nitrate (NO(3)(-)) using denitrifying bacteria. This tool offers a sensitive, rapid, and stable method for studying nitrogen metabolism in aquatic environments.

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Area of Science:

Environmental science
Biotechnology
Analytical chemistry

Background:

Nitrate (NO(3)(-)) is a crucial nutrient in aquatic ecosystems, but its accurate measurement can be challenging.
Existing methods for nitrate detection may have limitations in sensitivity, response time, or applicability to natural samples.

Purpose of the Study:

To develop and characterize a novel biosensor for the direct determination of nitrate concentrations.
To assess the performance of the biosensor in terms of sensitivity, selectivity, response time, and operational stability.

Main Methods:

Construction of a biosensor utilizing immobilized denitrifying bacteria lacking N(2)O reductase.
Quantification of nitrous oxide (N(2)O) produced from nitrate reduction using an electrochemical transducer.
Evaluation of sensor performance in freshwater and seawater across different temperatures.

Main Results:

The biosensor demonstrated linear responses to nitrate in both freshwater and seawater with a detection limit of approximately 1 μM.
Response times were rapid (15-60 s at room temperature).
The sensor operated for 2-4 days due to diffusive electron donor supply, with nitrite (NO(2)(-)) and nitrous oxide (N(2)O) identified as potential interferents.

Conclusions:

The developed NO(3)(-) biosensor is a valuable tool for in-situ monitoring of nitrate levels.
Its sensitivity, rapid response, and operational stability make it suitable for studying nitrogen metabolism in natural environments.
Further optimization may be needed to address interference from nitrite and nitrous oxide.