Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

Coagulation

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...

Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The effect of shadowed areas on smothering risk in three commercial free-range layer poultry farms, Australia 2019-2022.

Preventive veterinary medicine·2026

Same author

Risk factors for smothering in three commercial free-range layer poultry farms, Australia 2019-2022.

Preventive veterinary medicine·2025

Same author

Intracellular Nanodelivery of DNA with Enzyme-Degradable and pH-Responsive Peptide Dendrons.

Biomacromolecules·2025

Same author

Descriptive epidemiology of smothering in Australian commercial free-range layer hen farms.

Preventive veterinary medicine·2024

Same author

Perseverance rover reveals an ancient delta-lake system and flood deposits at Jezero crater, Mars.

Science (New York, N.Y.)·2021

Same author

Pre-slaughter factors linked to variation in responses to carbon dioxide gas stunning in pig abattoirs.

Animal : an international journal of animal bioscience·2021

Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026

Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026

Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026

Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026

Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026

Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Continuous-flow extraction of colloidal components in aqueous samples.

M Rice¹, J Roeraade, B Holmbom

¹Department of Analytical Chemistry, Royal Institute of Technology, S-100 44 Stockholm, Sweden.

Analytical Chemistry

|June 7, 2011

Summary

This summary is machine-generated.

This study introduces a new continuous-flow extraction system that enhances analyte recovery. By increasing mechanical energy and turbulence, the system significantly improves the extraction of colloidal analytes, achieving up to a 9-fold increase.

More Related Videos

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Related Experiment Videos

Last Updated: Jun 1, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Published on: October 4, 2012

Area of Science:

Analytical Chemistry
Environmental Science

Background:

Classical segmented-flow extraction shows limitations for hydrophobic colloidal analytes due to insufficient sample-solvent contact.
Improving extraction efficiency for colloidal substances in aqueous matrices is crucial for accurate chemical analysis.

Purpose of the Study:

To develop and evaluate a novel continuous-flow extraction system for dissolved and colloidal analytes.
To enhance the extraction efficiency of hydrophobic colloidal analytes in aqueous samples.
To investigate the impact of mechanical energy and turbulence on extraction performance.

Main Methods:

Development of a continuous-flow extraction system utilizing high-velocity extractant injection through a narrow-bore nozzle.
Generation of intense turbulence and fine droplet dispersion within the solvent stream.
High-speed photography with pulsed laser fluorescence to study flow dynamics.
Comparative analysis using a model sample of colloidal wood resin compounds in water.

Main Results:

The novel system demonstrated significantly improved extraction efficiency for colloidal triglycerides compared to classical methods.
An extraction enhancement of up to 9 times was observed for colloidal analytes.
Both dissolved and colloidal components showed good extraction, with notable gains for the latter.
High-speed imaging confirmed intense turbulence generation within the system.

Conclusions:

The developed continuous-flow extraction system effectively overcomes the limitations of classical methods for colloidal analytes.
Injecting extractant at high velocity to create turbulence is a key factor in enhancing extraction efficiency.
This technology offers a promising advancement for the analysis of complex aqueous samples containing both dissolved and colloidal substances.