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Updated: Jun 1, 2026

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Published on: August 22, 2018
Measurement of the binding of proteins to polyelectrolytes by frontal analysis continuous capillary electrophoresis
J Y Gao1, P L Dubin, B B Muhoberac
1Department of Chemistry, Indiana University [Formula: see text] Purdue University at Indianapolis, Indianapolis, Indiana 46202.
Abstract:
We have developed a novel technique, frontal analysis continuous capillary electrophoresis (FACCE), to study the binding of proteins to polyelectrolytes. Compared with existing electrophoresis methods such as conventional frontal analysis and the Hummel-Dreyer method, FACCE offers enhanced lower detection limits and is free from effects due to slow binding kinetics, thus making it suitable for studying equilibrium systems. In addition, with a single calibration, FACCE provides for efficient quantitative analysis. Here we report results obtained with β-lactoglobulin as the ligand and sodium poly(styrenesulfonate) (NaPSS) as the ligand-binding substrate. For this model system, FACCE yields reproducible calibration curves and binding isotherms. The binding parameters so determined are compared with previous results for other protein-polyelectrolyte systems.
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