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Laser Vaporization/Ionization Interface for Capillary Electrophoresis-Time-of-Flight Mass Spectrometry.

S Y Chang¹, E S Yeung

¹Ames Laboratory [Formula: see text] USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011.

Analytical Chemistry

|June 7, 2011

Summary

We developed a new laser vaporization/ionization time-of-flight mass spectrometry system for capillary electrophoresis (CE). This method efficiently analyzes biomolecules like amines and peptides, achieving a serotonin detection limit of 10(-7) M.

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Area of Science:

Analytical Chemistry
Biochemistry
Physical Chemistry

Background:

Capillary electrophoresis (CE) coupled with mass spectrometry (MS) is a powerful technique for biomolecule analysis.
Traditional CE-MS interfaces often require makeup solvent, complicating the system.
Existing ionization methods like electrospray ionization have limitations.

Purpose of the Study:

To introduce a novel laser vaporization/ionization time-of-flight mass spectrometry system for on-line CE analysis.
To demonstrate the compatibility of CE's low flow rates with direct MS interfacing.
To analyze small biomolecules, including amines and peptides, using the new CE/MS system.

Main Methods:

Development of a laser vaporization/ionization source for time-of-flight mass spectrometry.
On-line coupling of capillary electrophoresis (CE) with the developed mass spectrometry system.
Direct introduction of the total column effluent from CE into the MS system without makeup solvent.
Analysis of amine and peptide mixtures using the CE/MS system.

Main Results:

Successful implementation of a new laser vaporization/ionization MS system for CE.
Demonstrated high compatibility of CE's low flow rates (<1 μL/min) with direct MS interfacing.
Achieved a concentration detection limit of 10(-7) M for serotonin.
Separated and identified components within an amine mixture, showcasing the system's analytical capability.

Conclusions:

The novel laser vaporization/ionization CE/MS system offers an efficient and simplified approach for biomolecule analysis.
The system eliminates the need for makeup solvent, reducing complexity and potential contamination.
CE/MS analysis provides complementary information for the identification and separation of complex mixtures.