Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 1, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Two-channel sequential single-molecule measurement.

R D Guenard1, L A King, B W Smith

  • 1Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200.

Analytical Chemistry
|June 7, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An organotypic human melanoma-in-skin model as an <i>in vitro</i> tool for testing Vγ9Vδ2-T cell-based immunotherapy.

Immuno-oncology technology·2024
Same author

TURN-IT: a novel turning intervention program to improve quality of turning in daily life in people with Parkinson's disease.

BMC neurology·2022
Same author

Phosphorimetry.

Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2021
Same author

Clustered cases of infections due to an uncommon methicillin-resistant Staphylococcus aureus originating in a maternity ward.

Infectious diseases now·2021
Same author

Inflection points in longitudinal models: Tracking recovery and return to play following concussion.

Scandinavian journal of medicine & science in sports·2018
Same author

Metastatic placental site trophoblastic tumor.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2018
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

This study demonstrates sequential detection of single molecules using near-unity efficiency. This method shows great potential for rapid quantitative analysis of dilute solutions.

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Accurate detection of single molecules is crucial for quantitative analysis.
  • Traditional methods face challenges in efficiency and speed for dilute solutions.

Purpose of the Study:

  • To demonstrate sequential detection of single molecules with high measurement efficiency.
  • To validate the technique using autocorrelation, photobleaching, and cross-correlation analysis.
  • To assess the potential for rapid quantitative analysis of dilute solutions.

Main Methods:

  • Detection of IR140 dye molecules in sequential probe volumes within a capillary flow system.
  • Utilizing autocorrelation and photobleaching for single-molecule confirmation.
  • Employing cross-correlation analysis to verify sequential detection and flow dynamics.

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Related Experiment Videos

Last Updated: Jun 1, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Main Results:

  • Achieved near-unity measurement efficiency for sequential single-molecule detection.
  • Photobleaching data aligned with bulk photodestruction curves.
  • Cross-correlation analysis confirmed sequential detection with temporal delays consistent with parabolic flow.

Conclusions:

  • The developed method enables highly efficient sequential detection of single molecules.
  • This technique offers significant potential for rapid and accurate quantitative analysis of dilute samples.