Automatic cytometric device using multiple wavelength excitations
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Updated: Jun 1, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Nelly Rongeat1, Sylvain Ledroit, Laurence Chauvet
1HORIBA Medical, Parc Euromédecine, Rue du Caducée, 34000 Montpellier Cedex 4, France. nelly.rongeat@horiba.com
Abstract:
Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.
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