Development of an extended multilocus sequence typing for genotyping of Brucella isolates
Yanfen Chen1, Qing Zhen, Yufei Wang
1School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, PR China.
Journal of Microbiological Methods
|June 7, 2011
Abstract:
By amplifying and sequencing longer sequences, an extended multi locus sequence typing (EMLST) theme was developed for Brucella. 61 isolates were genotyped by the EMLST with increased resolution. This strategy could be extended to other bacteria to improve MLST genotyping resolution without additional loci.

