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10:57
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
A reagent-free method based on a photo-induced fluorimetry in a sequential injection system
Marieta L C Passos1, M Lúcia M F S Saraiva, João L M Santos
1REQUIMTE, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4090-030 Porto, Portugal.
Talanta
|June 7, 2011
Summary
A new automated method uses UV light and sequential injection analysis (SIA) for sensitive indomethacin determination in pharmaceuticals. This environmentally friendly approach offers improved analytical performance and accuracy for drug analysis.
Area of Science:
- Analytical Chemistry
- Green Chemistry
- Pharmaceutical Analysis
Background:
- Growing demand for environmentally friendly analytical methods.
- Need for reduced reagent consumption and enhanced analytical performance.
- Importance of accurate indomethacin determination in pharmaceutical formulations.
Purpose of the Study:
- To develop an automated, sensitive, and environmentally friendly method for indomethacin determination.
- To utilize sequential injection analysis (SIA) coupled with UV-induced fluorescence.
- To validate the method for pharmaceutical analysis, including dissolution testing.
Main Methods:
- Development of a sequential injection analysis (SIA) system.
- Utilizing a photoreactor with fluorimetric detection (λ(exc)=287 nm, λ(em)=378 nm).
- Application of ultra-violet (UV) light to enhance indomethacin fluorescence.
Main Results:
- Linear calibration for indomethacin from 4.10 × 10(-6) to 9.00 × 10(-5) mol L(-1).
- Achieved a low detection limit of 1.23 × 10(-6) mol L(-1).
- Demonstrated method reproducibility (R.S.D.<5%) and a sampling rate of ~20/hour, with no interference from common excipients.
Conclusions:
- The developed SIA method is suitable for sensitive and accurate indomethacin determination in pharmaceutical formulations.
- The methodology aligns with green chemistry principles by reducing reagent consumption.
- The method showed good agreement with established procedures in statistical evaluation.
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