Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions.

Nature communications·2025
Same author

Correction to "Development of BromoTag: A "Bump-and-Hole"-PROTAC System To Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins".

Journal of medicinal chemistry·2025
Same author

The Atad5 RFC-like complex is the major unloader of proliferating cell nuclear antigen in Xenopus egg extracts.

The Journal of biological chemistry·2023
Same author

The location and development of Replicon Cluster Domains in early replicating DNA.

Wellcome open research·2023
Same author

Overexpression of IκB⍺ modulates NF-κB activation of inflammatory target gene expression.

Frontiers in molecular biosciences·2023
Same author

Author Correction: Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.

Nature communications·2022

Related Experiment Video

Updated: Jun 1, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

How dormant origins promote complete genome replication.

J Julian Blow1, Xin Quan Ge, Dean A Jackson

  • 1Wellcome Trust Centre for Gene Regulation & Expression, University of Dundee Dow Street, Dundee DD1 5EH, UK. j.j.blow@dundee.ac.uk

Trends in Biochemical Sciences
|June 7, 2011
PubMed
Summary

Dormant origins safeguard genome integrity by activating when replication stalls. Suppressing new replication factories ensures timely DNA synthesis, preventing genomic instability and cancer.

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Related Experiment Videos

Last Updated: Jun 1, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Many licensed replication origins remain unused during G1 phase.
  • These dormant origins serve as a crucial backup for genome replication.

Purpose of the Study:

  • To review the function and regulation of dormant origins.
  • To discuss the physiological implications of dormant origin activation.

Main Methods:

  • Review of existing literature on DNA replication and genome stability.
  • Analysis of molecular mechanisms regulating dormant origin activation.
  • Examination of implications in mouse models with altered MCM2-7 levels.

Main Results:

  • Dormant origins activate upon replication fork stalling to maintain genome integrity.
  • DNA damage response kinases suppress new replication factory assembly during stress.
  • Reduced MCM2-7 levels lead to fewer dormant origins, increasing cancer proneness and genetic instability.

Conclusions:

  • Dormant origins are essential for preserving genome integrity under replication stress.
  • Their regulated activation is a key defense mechanism against genomic instability.
  • Dysregulation of dormant origins has significant implications for cancer and genetic instability.