Related Experiment Video
Updated: Jun 1, 2026
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Published on: February 27, 2014
Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with
Yan-Hong Wang1, Bin Ge, Xiao-Lai Yang
1Department of Pharmacy, People's Hospital of Gansu Province, Lanzhou 730000, Gansu, China.
Abstract:
The aim of this study was to elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE) on recurrent ulcerative colitis (UC) in rats. GSPE in doses of 100, 200, and 400mg/kg were intragastrically administered per day for 7 days after recurrent colitis was twice-induced by TNBS. The levels of GSH, as well as the activity of GSH-Px and SOD in colon tissues were measured by biochemical methods. The expression levels of tumor necrosis factor-α (TNF-α) and the nuclear translocation levels of nuclear factor-kappa B (NF-κB) in the colon tissues were measured by enzyme-linked immunosorbent assay methods. Western blotting analysis was used to determine the protein expression levels of inhibitory kappa B-alpha (IκBα), inhibitor kappa B kinase (IKKα/β), phosphorylated IκBα and phosphorylated IKKα/β. GSPE treatment was associated with a remarkable increased the activity of GSH-Px and SOD with GSH levels in TNBS-induced recurrent colitis rats as compared to the model group. GSPE also significantly reduced the expression levels of TNF-α, p-IKKα/β, p-IκBα and the translocation of NF-κB in the colon mucosa. GSPE exerted a protective effect on recurrent colitis in rats by modifying the inflammatory response and promoting damaged tissue repair to improve colonic oxidative stress. Moreover, GSPE inhibited the TNBS-induced inflammatory of recurrent colitis though blocking NF-κB signaling pathways.
Insights
Grape seed proanthocyanidins (GSP) show therapeutic effects on recurrent ulcerative colitis in rats. GSPE reduces inflammation and oxidative stress by blocking NF-κB signaling pathways.
Area of Science:
- Gastroenterology
- Pharmacology
- Molecular Biology
Background:
- Ulcerative colitis (UC) is a chronic inflammatory bowel disease.
- Recurrent UC poses significant therapeutic challenges.
- Grape seed proanthocyanidins (GSPE) are investigated for their anti-inflammatory properties.
Purpose of the Study:
- To elucidate the molecular mechanisms of GSPE in treating TNBS-induced recurrent colitis in rats.
- To evaluate the impact of GSPE on oxidative stress and inflammatory markers.
- To investigate the role of NF-κB signaling in GSPE's therapeutic effects.
Main Methods:
- TNBS-induced recurrent colitis model in rats.
- Intragastric administration of GSPE (100, 200, 400 mg/kg).
- Biochemical assays for GSH, GSH-Px, and SOD.
- ELISA for TNF-α and NF-κB.
- Western blotting for IκBα, IKKα/β, and their phosphorylated forms.
Main Results:
- GSPE significantly increased GSH levels and GSH-Px and SOD activity.
- GSPE markedly reduced TNF-α expression and NF-κB nuclear translocation.
- GSPE inhibited the phosphorylation of IKKα/β and IκBα, indicating NF-κB pathway blockade.
Conclusions:
- GSPE demonstrates protective effects against recurrent colitis in rats.
- GSPE ameliorates inflammation and oxidative stress by inhibiting the NF-κB signaling pathway.
- GSPE promotes tissue repair and reduces colonic oxidative stress in UC models.
Related Concept Videos
02:26NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:20Inflammatory Bowel Disease II: Ulcerative Colitis