Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

4‑Aminoalkylquinolines as Potent Antitubercular Agents Targeting the Cytochrome bc<sub>1</sub> Complex.

ACS medicinal chemistry letters·2026
Same author

Guanilated Quinolones with Dual Antitubercular and Anti-Inflammatory Activities.

ChemMedChem·2026
Same author

Structure-Activity relationship and optimization of drug-like properties of antituberculosis 3-(4,4-dimethyl-1,4-azasilinane)methylpyrazole MmpL3 inhibitors.

European journal of medicinal chemistry·2026
Same author

Trends in primary brain tumour incidence and mortality in Ireland 1995-2019.

Irish journal of medical science·2026
Same author

Integrating chemical, genetic, and feasibility assessments for anti-tubercular target validation.

EMBO molecular medicine·2026
Same author

Prospective randomised trial comparing thermal ablation With laparoscopic Adrenalectomy as an alternatiVE treatment for unilateral asymmetric primary aldosteronism: a protocol for the WAVE trial.

BMJ open·2026

Related Experiment Video

Updated: Jun 1, 2026

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach.

Paul Carroll1, Marie-Claire Faray-Kele, Tanya Parish

  • 1Queen Mary University of London, Barts and the London, London E1 2AT, United Kingdom.

Applied and Environmental Microbiology
|June 7, 2011
PubMed
Summary

Researchers identified key drug targets in Mycobacterium tuberculosis by downregulating specific genes. The dprE1, clpP1, and fadD32 operons emerged as the most promising targets for new tuberculosis therapies.

More Related Videos

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

Related Experiment Videos

Last Updated: Jun 1, 2026

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

Area of Science:

  • Microbiology
  • Genetics
  • Drug Discovery

Background:

  • Mycobacterium tuberculosis (M. tuberculosis) is the causative agent of tuberculosis.
  • Identifying novel drug targets is crucial for combating drug-resistant strains.

Purpose of the Study:

  • To identify vulnerable drug targets in M. tuberculosis.
  • To rank potential drug targets based on their essentiality for bacterial growth.

Main Methods:

  • Construction of recombinant M. tuberculosis strains with downregulated gene expression.
  • Assessment of growth phenotypes in both macrophage infection models and standard culture conditions.

Main Results:

  • The dprE1, clpP1, and fadD32 operons were identified as highly effective drug targets.
  • Genes such as glnA1, glnE, pknL, regX3, and senX3 were found to be poor drug targets.

Conclusions:

  • The study highlights the dprE1, clpP1, and fadD32 operons as critical targets for M. tuberculosis.
  • This research provides a foundation for developing new anti-tuberculosis drugs targeting essential pathways.