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Updated: Jun 1, 2026

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Published on: March 24, 2014
Sharper low-power STED nanoscopy by time gating
Giuseppe Vicidomini1, Gael Moneron, Kyu Y Han
1Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.
Abstract:
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

