Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuropathy-predominant and hyperkinetic-dystonic presentations in two siblings with a homozygous VPS13A variant: long-term follow-up and family-based clinical analysis.

BMC neurology·2026
Same author

Deep learning-assisted structural color inverse design: a perspective.

Chemical science·2026
Same author

Safety evaluation of subcutaneous and intravenous administration of infliximab: a real-world study based on the FAERS database.

Frontiers in medicine·2026
Same author

Imprinted high-<i>Q</i> polymer micro-ring resonator array for high-resolution photoacoustic tomography.

Opto-electronic advances·2026
Same author

Repeated intravenous weight-based ustekinumab maintenance as an optimization strategy for Crohn's disease patients with loss of response to standard therapy.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2026
Same author

Association of the Stress Hyperglycemia Ratio with Advanced Liver Fibrosis and Mortality in Patients with Metabolic Dysfunction-Associated Steatotic Liver Disease: An Analysis of NHANES.

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology·2026

Related Experiment Video

Updated: Jun 1, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Pure optical photoacoustic microscopy.

Zhixing Xie1, Sung-Liang Chen, Tao Ling

  • 1Department of Radiology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Optics Express
|June 7, 2011
PubMed
Summary

Pure optical photoacoustic microscopy (POPAM) uses a microring resonator for high-resolution imaging. This novel technique achieves superior sensitivity and resolution for visualizing microvasculature and red blood cells.

More Related Videos

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Jun 1, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Acoustic Sensing

Background:

  • Conventional photoacoustic microscopy (PAM) is limited by detector bandwidth, impacting axial resolution.
  • Existing optical resonant structures for acoustic measurement lack the sensitivity required for microscale imaging.

Purpose of the Study:

  • To introduce and validate a novel Pure Optical Photoacoustic Microscopy (POPAM) system.
  • To demonstrate the capability of POPAM for high-resolution imaging of microvasculature and cellular structures.

Main Methods:

  • Fabrication of a high-Q factor microring resonator using nanoimprinting.
  • Utilizing optical rastering of a focused excitation beam for signal generation.
  • Optically sensing photoacoustic signals with the microring resonator.

Main Results:

  • Achieved an ultrahigh Q factor of 3.0×10^5, resulting in a noise equivalent detectable pressure (NEDP) of 29 Pa.
  • Demonstrated lateral resolution of 5 micrometers and axial resolution of 8 micrometers.
  • Successfully visualized 3D microvasculature, capillary networks, and individual red blood cells in ex vivo and in vivo mouse models.

Conclusions:

  • POPAM offers high sensitivity and resolution, complementing existing optical microscopy modalities.
  • The system provides unique optical absorption contrast and functional information.
  • The technology holds potential for higher resolution imaging in thicker tissue samples than currently possible.