Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessment of dried blood spot and umbilical cord blood samples for the quantification of maternally transferred IgG against the group B streptococcal alpha-like surface proteins in neonates at Kolding Hospital, Denmark: a proof-of-concept, cross-sectional study.

The Lancet. Microbe·2026

Same author

The overlooked role of the seminovaginal microbiota in infertility: a narrative mini review.

Frontiers in medicine·2026

Same author

Role of bacterial dysbiosis in infertility: the BIOME study protocol for a Danish multicentre prospective observational cohort study.

BMJ open·2026

Same author

Antibiotic Treatment Duration for Salmonella Osteoarticular Infections in Children - A Systematic Review and Comparative Study.

Acta paediatrica (Oslo, Norway : 1992)·2026

Same author

The effect of the probiotic supplement-ASTARTE™-on the reduction of recurrent urinary tract infection in women aged 18-40 years: protocol for a randomized, double-blind, placebo-controlled study.

Trials·2026

Same author

A nationwide seroprevalence study of Francisella tularensis in Denmark.

Ticks and tick-borne diseases·2026

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Optimising bacterial DNA extraction from faecal samples: comparison of three methods.

Birgitte Smith¹, Nan Li, Anders Schou Andersen

¹Department of Microbiological Surveillance and Research, Statens Serum Institut, DK-2300 S, Copenhagen, Denmark.

The Open Microbiology Journal

|June 7, 2011

Summary

Mechanical disruption of fecal samples using TissueLyser or Mini BeadBeater 8 enhances bacterial DNA extraction for infectious disease diagnostics. Standard kits alone yield fewer bacterial DNA bands.

Keywords:

DNA extraction method Mini BeadBeater 8 TissueLyser.faecal samples

More Related Videos

DNA Viral Size Fraction Metagenomics for Human Stool Samples

DNA Viral Size Fraction Metagenomics for Human Stool Samples

Published on: March 24, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Related Experiment Videos

Last Updated: Jun 1, 2026

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

DNA Viral Size Fraction Metagenomics for Human Stool Samples

DNA Viral Size Fraction Metagenomics for Human Stool Samples

Published on: March 24, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Area of Science:

Microbiology
Molecular Biology
Diagnostic Laboratory Science

Background:

Culture-independent methods are crucial for infectious disease diagnostics.
Genomic DNA isolation from clinical samples, especially feces, is a critical first step.
Mechanical cell disruption methods for fecal DNA extraction are varied, with limited prior research on specific devices like TissueLyser.

Purpose of the Study:

To evaluate and implement an optimal method for preparing human fecal samples for DNA extraction.
To compare the efficacy of different mechanical disruption techniques prior to DNA purification.

Main Methods:

Three DNA extraction procedures for human fecal samples were compared.
Methods included: 1) Mini BeadBeater 8 with QIAamp DNA stool MiniKit, 2) TissueLyser with QIAamp DNA stool MiniKit, and 3) QIAamp DNA stool MiniKit alone.
DNA yield and diversity were analyzed using Denaturing Gradient Gel Electrophoresis (DGGE).

Main Results:

No significant difference in the number of bacterial DNA bands was observed between the TissueLyser and Mini BeadBeater 8 methods.
Both mechanical disruption methods yielded a greater number of bacterial DNA bands compared to using the QIAamp DNA stool MiniKit without prior mechanical disruption.
The QIAamp DNA stool MiniKit alone resulted in a reduced number of detectable bacterial bands.

Conclusions:

TissueLyser and Mini BeadBeater 8 are comparable and effective mechanical disruptors for isolating bacterial DNA from fecal samples.
Mechanical disruption significantly improves DNA extraction efficiency for fecal samples compared to standard kit procedures alone.
Optimized DNA extraction methods are essential for accurate results in culture-independent diagnostic applications.