Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
Inflammation01:38

Inflammation

Overview
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
Lipid Absorption01:24

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lecithin:cholesterol acyltransferase binds a discontinuous binding site on adjacent apolipoprotein A-I belts in HDL.

Journal of lipid research·2025
Same author

Conformational flexibility of apolipoprotein A-I amino- and carboxy-termini is necessary for lipid binding but not cholesterol efflux.

Journal of lipid research·2022
Same author

Macrophage SR-BI modulates autophagy via VPS34 complex and PPARα transcription of Tfeb in atherosclerosis.

The Journal of clinical investigation·2021
Same author

2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy.

Scientific reports·2020
Same author

7-Ketocholesterol in disease and aging.

Redox biology·2020
Same author

Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study.

eLife·2018

Related Experiment Video

Updated: Jun 1, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

Lysosomes, cholesterol and atherosclerosis.

W Gray Jerome1

  • 1Department of Pathology, U-2206 Medical Center North Vanderbilt University School of Medicine 1161 21st Avenue, South Nashville, TN 37232-32561, USA, Tel.: +1 615 322 5530.

Clinical Lipidology
|June 7, 2011
PubMed
Summary
This summary is machine-generated.

Triglyceride particles can reduce cholesterol in macrophage foam cells, improving lysosome function and potentially mitigating atherosclerosis. This approach offers a novel strategy for managing sterol buildup in these critical cells.

More Related Videos

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Related Experiment Videos

Last Updated: Jun 1, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Area of Science:

  • Cardiovascular Biology
  • Cellular Metabolism
  • Atherosclerosis Research

Background:

  • Macrophage foam cells are central to atherosclerotic lesions.
  • Lysosomal sterol accumulation impairs foam cell function and promotes disease.
  • Mobilizing sterols from lysosomes is a significant therapeutic challenge.

Purpose of the Study:

  • To investigate the effects of triglyceride-containing particles on lysosomal sterol accumulation in foam cells.
  • To explore potential mechanisms by which triglycerides impact lysosomal cholesterol.
  • To evaluate the therapeutic implications of reducing lysosomal sterol burden.

Main Methods:

  • Treatment of cultured, cholesterol-engorged macrophages with triglyceride-rich particles.
  • Assessment of lysosomal function and sterol content post-treatment.
  • Review of existing literature on lysosomal sterol metabolism and foam cell biology.

Main Results:

  • Triglyceride particle treatment reversed detrimental effects of cholesterol on lysosomes.
  • Significant reduction in cellular sterol burden was observed.
  • Potential for improved lysosome function and cell viability.

Conclusions:

  • Triglyceride-containing particles show promise in reducing lysosomal cholesterol in foam cells.
  • This strategy may offer a novel therapeutic avenue for atherosclerosis.
  • Further investigation into mechanisms and clinical effects is warranted.