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Characterization of avian reovirus strain-specific polymorphisms
F D Clark1, Y Ni, E W Collisson
1Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
Avian Diseases
|April 1, 1990
Summary
Avian reoviruses exhibit diverse pathogenic properties, causing transient digestive system disorders or viral arthritis syndrome in chickens. Genome analysis revealed extreme genetic diversity, preventing genotype-pathotype correlation but offering insights into viral pathogenesis.
Area of Science:
- * Veterinary Virology
- * Molecular Biology
- * Avian Pathology
Background:
- * Avian reoviruses are linked to various poultry diseases.
- * Genotype-disease correlations remain unestablished.
- * Understanding viral genetic diversity is crucial for pathogenesis studies.
Purpose of the Study:
- * To compare the pathogenic properties of six avian reovirus strains in chickens.
- * To analyze the electrophoretic mobility of viral genomic segments and encoded proteins.
- * To investigate potential correlations between avian reovirus genotypes and disease manifestations.
Main Methods:
- * Peroral and footpad inoculation of chickens with six avian reovirus strains.
- * Electrophoretic analysis of viral genomic segments.
- * Comparative analysis of viral protein electrophoretic mobility.
Main Results:
- * Three distinct pathotypes identified: Pathotype I (TDSD), Pathotype II (VAS), and Pathotype III (TDSD and VAS).
- * Avian reovirus genomes demonstrated extreme polymorphism, with significant variations in segment co-migration.
- * Pronounced variations observed in the electrophoretic mobility and molecular size of viral proteins, including the sigma 4 attachment protein.
Conclusions:
- * Extreme genomic polymorphism in avian reoviruses prevents direct genotype-pathotype correlation.
- * The study provides essential genetic elements for characterizing gene functions in viral pathogenesis.
- * Further research is needed to elucidate specific genetic determinants of avian reovirus disease.