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Related Experiment Video

Updated: Jun 1, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Fast aerosol analysis by fourier transform imaging fluorescence microscopy.

M Fisher¹, V Bulatov, S Hasson

¹Department of Chemistry, Technion [Formula: see text] Israel Institute of Technology, Haifa 32000, Israel.

Analytical Chemistry

|June 8, 2011

Summary

This study introduces a novel Fourier transform imaging spectroscopy method for rapid analysis of airborne polycyclic aromatic hydrocarbons (PAHs). The technique offers high sensitivity for mapping and quantifying PAH contamination on aerosols.

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Area of Science:

Environmental Science
Analytical Chemistry
Spectroscopy

Background:

Polycyclic aromatic hydrocarbons (PAHs) are significant air pollutants.
Accurate and rapid detection of airborne PAHs is crucial for environmental monitoring.
Existing methods for PAH analysis can be time-consuming or lack spatial resolution.

Purpose of the Study:

To develop and validate a fast, sensitive imaging technique for analyzing airborne PAHs.
To map and quantify PAH contamination on collected aerosol samples.
To assess the potential for in situ and on-line monitoring of particulate PAHs.

Main Methods:

Combining Fourier transform imaging spectroscopy with fluorescence microscopy and a cooled CCD detector.
Collecting aerosols on glass fiber filters for analysis.
Utilizing the technique to obtain a full fluorescence spectrum at each pixel for spatial mapping.
Developing calibration plots for quantification.

Main Results:

Achieved quantification limits in the range of 10 ng cm(-2) on filter (20 ng m(-3) in air) with 1-minute integration.
Estimated an absolute detection limit as low as 0.25 pg on filter (0.5 pg m(-3) in air) with 1-minute integration.
Demonstrated the method's capability for analyzing monocomponent contamination and simple mixtures.

Conclusions:

Fourier transform imaging spectroscopy provides a powerful tool for rapid PAH analysis in aerosols.
The method shows potential for in situ and on-line monitoring of airborne PAH contamination.
Further automation could enable real-time environmental monitoring applications.