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Laser desorption-membrane introduction mass spectrometry.

M H Soni1, J H Callahan, S W McElvany

  • 1Analytical Chemistry Section, Code 6113, U.S. Naval Research Laboratory, Washington, D.C. 20375.

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|June 8, 2011
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This study introduces laser desorption with membrane introduction mass spectrometry (MIMS) for analyzing challenging compounds. This novel technique enhances sensitivity and speed for high molecular weight, low volatility analytes in aqueous solutions.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Spectroscopy

Background:

  • Membrane Introduction Mass Spectrometry (MIMS) is a technique for analyzing aqueous solutions.
  • Traditional MIMS faces limitations in sensitivity and response time for high molecular weight and low volatility compounds.
  • Laser desorption offers a potential method to overcome these limitations.

Purpose of the Study:

  • To describe the first application of laser desorption coupled with MIMS.
  • To investigate the effectiveness of this combined technique for analyzing challenging analytes.
  • To develop and demonstrate interfaces for integrating laser desorption into MIMS analysis.

Main Methods:

  • Utilized a low-powered carbon dioxide laser to irradiate the vacuum side of a silicone membrane during MIMS analysis.
  • Developed two interfaces for performing laser desorption within and outside the GCQ ion trap mass spectrometer vacuum manifold.
  • Employed flow injection (FI) for sample introduction of aqueous solutions.

Main Results:

  • Demonstrated direct on-line monitoring of high boiling point (200-530 °C) polynuclear aromatic hydrocarbons (PAHs).
  • Achieved improved sensitivity and response times for analytes like naphthalene, anthracene, pyrene, benzo[b]fluoranthene, and indeno[123-cd]pyrene.
  • Laser absorption led to direct membrane heating and rapid desorption of permeate molecules.

Conclusions:

  • Laser desorption-MIMS is a viable technique for enhancing the analysis of low volatility compounds in aqueous solutions.
  • The developed interfaces facilitate the integration of laser desorption into existing mass spectrometry setups.
  • This method offers a significant advancement for the on-line monitoring of complex organic pollutants.