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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Published on: September 10, 2014

Solvent-programmed microchip open-channel electrochromatography.

J P Kutter1, S C Jacobson, N Matsubara

  • 1Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142.

Analytical Chemistry
|June 8, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces open-channel electrochromatography on a microchip for rapid separation of neutral dyes. Optimized channel depths and computer-controlled solvent gradients achieve efficient analysis in under 60 seconds.

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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Open-channel electrochromatography (OCE) offers potential for miniaturized separations.
  • Microchip devices enable integrated sample handling and analysis.
  • Solvent programming is crucial for optimizing chromatographic performance.

Purpose of the Study:

  • To demonstrate open-channel electrochromatography on a microchip device.
  • To investigate the effect of channel depth on chromatographic efficiency.
  • To utilize solvent programming for enhanced separation control.

Main Methods:

  • Microchip fabrication with octadecylsilane coated channels.
  • Implementation of electroosmotic flow for species transport and on-chip mixing.
  • Computer-controlled voltage application for isocratic and gradient elution.
  • Evaluation of different channel depths (10.2 to 2.9 μm) and van Deemter analysis.

Main Results:

  • Successful chromatographic separation of neutral dyes using OCE on a microchip.
  • Optimal channel depth identified around 5 μm for efficiency and operational ease.
  • Computer-controlled linear solvent gradients effectively tuned selectivity and reduced analysis time.
  • Complete separation runs achieved in under 60 seconds with excellent efficiencies, even with steep gradients.

Conclusions:

  • Microchip-based open-channel electrochromatography with solvent programming is a viable and efficient separation technique.
  • Channel depth is a critical parameter influencing separation performance.
  • Automated gradient control provides powerful means to optimize complex separations rapidly.